The following activities are prohibited within the Tortugas marine reserves: Fishing for any species and anchoring by fishing vessels.
(a) EEZ portion of Tortugas North. The area is bounded by rhumb lines connecting the following points: From point A at 24°40′00″ N. lat., 83°06′00″ W. long. to point B at 24°46′00″ N. lat., 83°06′00″ W. long. to point C at 24°46′00″ N. lat., 83°00′00″ W. long.; thence along the line denoting the seaward limit of Florida's waters, as shown on the current edition of NOAA chart 11438, to point A at 24°40′00″ N. lat., 83°06′00″ W. long.
(b) Tortugas South. The area is bounded by rhumb lines connecting, in order, the following points:
| Point | North lat. | West long. |
|---|---|---|
| A | 24°33′00″ | 83°09′00″ |
| B | 24°33′00″ | 83°05′00″ |
| C | 24°18′00″ | 83°05′00″ |
| D | 24°18′00″ | 83°09′00″ |
| A | 24°33′00″ | 83°09′00″ |